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e13 5 cd1 mouse embryos  (Inotiv)


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    Structured Review

    Inotiv e13 5 cd1 mouse embryos
    DRG <t>from</t> <t>E13.5</t> mice were dissociated and grown in the presence of NGF for 2 days. The glial cells were then transfected with plasmids expressing MEGF10::GFP; Jedi-1::Flag, or meGFP and NGF removed from the cultures. The cells were fixed after 2 days without NGF and immunolabeled with antibodies to GFP or Flag. (a and b) Z-axis optical stacks of confocal images of glial cells expressing MEGF10::GFP (M10::GFP; a, c and d), Jedi-1::Flag (Jd::Flag; b, c and d), or meGFP (c and d) were acquired and the numbers of transfected cells containing at least one ingested nuclear remnant [condensed TO-PRO3 staining] was quantified. Arrows indicate the location of engulfed apoptotic nuclei in these cells. Arrowheads indicate the long processes in Jedi-1::Flag expressing cells. In (c) the error bars = Mean ± s.d. P=0.0002, one-way ANOVA. (d) The number of engulfed nuclei per engulfing cell was also determined and expressed as the percent of transfected cells containing the indicated number of apoptotic nuclei (open bar: meGFP; black bar: MEGF10-GFP; gray bar: Jedi-Flag). Based on a chi-squared analysis, there was a significant difference between all 3 groups of cells (expressing Jedi-1, MEGF10 and meGFP. p<0.0001). Scale bars, 20 µm.
    E13 5 Cd1 Mouse Embryos, supplied by Inotiv, used in various techniques. Bioz Stars score: 99/100, based on 13506 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e13 5 cd1 mouse embryos/product/Inotiv
    Average 99 stars, based on 13506 article reviews
    e13 5 cd1 mouse embryos - by Bioz Stars, 2026-05
    99/100 stars

    Images

    1) Product Images from "Glial Precursors Clear Sensory Neuron Corpses during Development via Jedi-1, an Engulfment Receptor"

    Article Title: Glial Precursors Clear Sensory Neuron Corpses during Development via Jedi-1, an Engulfment Receptor

    Journal: Nature neuroscience

    doi: 10.1038/nn.2446

    DRG from E13.5 mice were dissociated and grown in the presence of NGF for 2 days. The glial cells were then transfected with plasmids expressing MEGF10::GFP; Jedi-1::Flag, or meGFP and NGF removed from the cultures. The cells were fixed after 2 days without NGF and immunolabeled with antibodies to GFP or Flag. (a and b) Z-axis optical stacks of confocal images of glial cells expressing MEGF10::GFP (M10::GFP; a, c and d), Jedi-1::Flag (Jd::Flag; b, c and d), or meGFP (c and d) were acquired and the numbers of transfected cells containing at least one ingested nuclear remnant [condensed TO-PRO3 staining] was quantified. Arrows indicate the location of engulfed apoptotic nuclei in these cells. Arrowheads indicate the long processes in Jedi-1::Flag expressing cells. In (c) the error bars = Mean ± s.d. P=0.0002, one-way ANOVA. (d) The number of engulfed nuclei per engulfing cell was also determined and expressed as the percent of transfected cells containing the indicated number of apoptotic nuclei (open bar: meGFP; black bar: MEGF10-GFP; gray bar: Jedi-Flag). Based on a chi-squared analysis, there was a significant difference between all 3 groups of cells (expressing Jedi-1, MEGF10 and meGFP. p<0.0001). Scale bars, 20 µm.
    Figure Legend Snippet: DRG from E13.5 mice were dissociated and grown in the presence of NGF for 2 days. The glial cells were then transfected with plasmids expressing MEGF10::GFP; Jedi-1::Flag, or meGFP and NGF removed from the cultures. The cells were fixed after 2 days without NGF and immunolabeled with antibodies to GFP or Flag. (a and b) Z-axis optical stacks of confocal images of glial cells expressing MEGF10::GFP (M10::GFP; a, c and d), Jedi-1::Flag (Jd::Flag; b, c and d), or meGFP (c and d) were acquired and the numbers of transfected cells containing at least one ingested nuclear remnant [condensed TO-PRO3 staining] was quantified. Arrows indicate the location of engulfed apoptotic nuclei in these cells. Arrowheads indicate the long processes in Jedi-1::Flag expressing cells. In (c) the error bars = Mean ± s.d. P=0.0002, one-way ANOVA. (d) The number of engulfed nuclei per engulfing cell was also determined and expressed as the percent of transfected cells containing the indicated number of apoptotic nuclei (open bar: meGFP; black bar: MEGF10-GFP; gray bar: Jedi-Flag). Based on a chi-squared analysis, there was a significant difference between all 3 groups of cells (expressing Jedi-1, MEGF10 and meGFP. p<0.0001). Scale bars, 20 µm.

    Techniques Used: Transfection, Expressing, Immunolabeling, Staining



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    e13 5 cd1 mouse embryos  (Inotiv)
    99
    Inotiv e13 5 cd1 mouse embryos
    DRG <t>from</t> <t>E13.5</t> mice were dissociated and grown in the presence of NGF for 2 days. The glial cells were then transfected with plasmids expressing MEGF10::GFP; Jedi-1::Flag, or meGFP and NGF removed from the cultures. The cells were fixed after 2 days without NGF and immunolabeled with antibodies to GFP or Flag. (a and b) Z-axis optical stacks of confocal images of glial cells expressing MEGF10::GFP (M10::GFP; a, c and d), Jedi-1::Flag (Jd::Flag; b, c and d), or meGFP (c and d) were acquired and the numbers of transfected cells containing at least one ingested nuclear remnant [condensed TO-PRO3 staining] was quantified. Arrows indicate the location of engulfed apoptotic nuclei in these cells. Arrowheads indicate the long processes in Jedi-1::Flag expressing cells. In (c) the error bars = Mean ± s.d. P=0.0002, one-way ANOVA. (d) The number of engulfed nuclei per engulfing cell was also determined and expressed as the percent of transfected cells containing the indicated number of apoptotic nuclei (open bar: meGFP; black bar: MEGF10-GFP; gray bar: Jedi-Flag). Based on a chi-squared analysis, there was a significant difference between all 3 groups of cells (expressing Jedi-1, MEGF10 and meGFP. p<0.0001). Scale bars, 20 µm.
    E13 5 Cd1 Mouse Embryos, supplied by Inotiv, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e13 5 cd1 mouse embryos/product/Inotiv
    Average 99 stars, based on 1 article reviews
    e13 5 cd1 mouse embryos - by Bioz Stars, 2026-05
    99/100 stars
    		  Buy from Supplier
    e13 5 cd1 mouse embryos  (Charles River Laboratories)
    86
    Charles River Laboratories e13 5 cd1 mouse embryos
    DRG <t>from</t> <t>E13.5</t> mice were dissociated and grown in the presence of NGF for 2 days. The glial cells were then transfected with plasmids expressing MEGF10::GFP; Jedi-1::Flag, or meGFP and NGF removed from the cultures. The cells were fixed after 2 days without NGF and immunolabeled with antibodies to GFP or Flag. (a and b) Z-axis optical stacks of confocal images of glial cells expressing MEGF10::GFP (M10::GFP; a, c and d), Jedi-1::Flag (Jd::Flag; b, c and d), or meGFP (c and d) were acquired and the numbers of transfected cells containing at least one ingested nuclear remnant [condensed TO-PRO3 staining] was quantified. Arrows indicate the location of engulfed apoptotic nuclei in these cells. Arrowheads indicate the long processes in Jedi-1::Flag expressing cells. In (c) the error bars = Mean ± s.d. P=0.0002, one-way ANOVA. (d) The number of engulfed nuclei per engulfing cell was also determined and expressed as the percent of transfected cells containing the indicated number of apoptotic nuclei (open bar: meGFP; black bar: MEGF10-GFP; gray bar: Jedi-Flag). Based on a chi-squared analysis, there was a significant difference between all 3 groups of cells (expressing Jedi-1, MEGF10 and meGFP. p<0.0001). Scale bars, 20 µm.
    E13 5 Cd1 Mouse Embryos, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e13 5 cd1 mouse embryos/product/Charles River Laboratories
    Average 86 stars, based on 1 article reviews
    e13 5 cd1 mouse embryos - by Bioz Stars, 2026-05
    86/100 stars
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    Image Search Results


    DRG from E13.5 mice were dissociated and grown in the presence of NGF for 2 days. The glial cells were then transfected with plasmids expressing MEGF10::GFP; Jedi-1::Flag, or meGFP and NGF removed from the cultures. The cells were fixed after 2 days without NGF and immunolabeled with antibodies to GFP or Flag. (a and b) Z-axis optical stacks of confocal images of glial cells expressing MEGF10::GFP (M10::GFP; a, c and d), Jedi-1::Flag (Jd::Flag; b, c and d), or meGFP (c and d) were acquired and the numbers of transfected cells containing at least one ingested nuclear remnant [condensed TO-PRO3 staining] was quantified. Arrows indicate the location of engulfed apoptotic nuclei in these cells. Arrowheads indicate the long processes in Jedi-1::Flag expressing cells. In (c) the error bars = Mean ± s.d. P=0.0002, one-way ANOVA. (d) The number of engulfed nuclei per engulfing cell was also determined and expressed as the percent of transfected cells containing the indicated number of apoptotic nuclei (open bar: meGFP; black bar: MEGF10-GFP; gray bar: Jedi-Flag). Based on a chi-squared analysis, there was a significant difference between all 3 groups of cells (expressing Jedi-1, MEGF10 and meGFP. p<0.0001). Scale bars, 20 µm.

    Journal: Nature neuroscience

    Article Title: Glial Precursors Clear Sensory Neuron Corpses during Development via Jedi-1, an Engulfment Receptor

    doi: 10.1038/nn.2446

    Figure Lengend Snippet: DRG from E13.5 mice were dissociated and grown in the presence of NGF for 2 days. The glial cells were then transfected with plasmids expressing MEGF10::GFP; Jedi-1::Flag, or meGFP and NGF removed from the cultures. The cells were fixed after 2 days without NGF and immunolabeled with antibodies to GFP or Flag. (a and b) Z-axis optical stacks of confocal images of glial cells expressing MEGF10::GFP (M10::GFP; a, c and d), Jedi-1::Flag (Jd::Flag; b, c and d), or meGFP (c and d) were acquired and the numbers of transfected cells containing at least one ingested nuclear remnant [condensed TO-PRO3 staining] was quantified. Arrows indicate the location of engulfed apoptotic nuclei in these cells. Arrowheads indicate the long processes in Jedi-1::Flag expressing cells. In (c) the error bars = Mean ± s.d. P=0.0002, one-way ANOVA. (d) The number of engulfed nuclei per engulfing cell was also determined and expressed as the percent of transfected cells containing the indicated number of apoptotic nuclei (open bar: meGFP; black bar: MEGF10-GFP; gray bar: Jedi-Flag). Based on a chi-squared analysis, there was a significant difference between all 3 groups of cells (expressing Jedi-1, MEGF10 and meGFP. p<0.0001). Scale bars, 20 µm.

    Article Snippet: DRG neurons from E13.5 CD1 mouse embryos were isolated as described above, plated on collagen coated coverslips and grown in UltraCULTURE media with 50 ng/ml NGF (Harlan).

    Techniques: Transfection, Expressing, Immunolabeling, Staining